Site-directed insertion of transgenes (Topics in Current Genetics Book 23) Book Features
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Im stuck with retakes for two courses of micro biology, while my passion lies more with ecology. I suck so hard at this and you really make my life less difficult. Thank you Shomu Thank you very much ! love from poland lots of salutations, i will pass my exam thanks to u and IgA and OG Anyone trying to pass 12th class biotechnology ? tysm ly Shomu Bosdi k English m likdo or Hindi m explain kardey Tq sir sorry its 5:47* sir sir i have a doubt that in the video at around 4:28 you have said that its different primer sequencing and is not same as PCR but again at last of the video you had presented it as a PCR method..... is it a PCR based primer designing method or no? Dada so mutagenesis is artificial? Thankyou so much sir How do i set the thermocycler to achieve this mutation? How will u take the mutated plasmid Loved it Wherever my professor lacks, an Indian man will step up and teach me. Good Thank you so much... Your way of reaching is amazing....really💞 Why is there a nick?? you must have gone to that, I mean replicating a circular piece of DNA, Thanku thanku thanku thanku thanku soooooo muchhhh sir 🙏🏻🙏🏻🙏🏻🙏🏻 where is password Sir aapnai kahi pr bhi password nhi btya PDF ka. Really amazing video
Thanks 😊😊😊😊 Sir ji bahut he acha video ha Nice vdo💫✨ Sir pls mke a video on PCR based methods of site directed mutagenesis Excellent video sir jii🙏🙏🙏 Excellent sir👌 Nice video's
Explain very well 💯💯💯 very clear explanation Thanks sir Very helpful thank you sir for your efforts 🙏🙏 Thank you sir Thank you sir, for such conceptual video Lot thanks sir Amazing video sir Awsm video sir Very helpful video sir Excellent video Sir ji
👍👍👍👍👍👍👍 Thanks sir ❤️❤️❤️❤️❤️ Awasome video and explanation Love your lectures. it's really easy to understand! Thank you so much! You are a superhero Everytime he finishes explaining something its like revealing the trick in the magic trick I wish you were my teacher all the time Beautifully explained by friend can someone assist me here, at exactly 8:31 he mentions that dna polymerase attaches at the 5' and starts synthesizing the template going in the 3' direction but i read somewhere that dna polymerase does not bind to the 5' site because it is INACTIVE, is this wrong? Thanks so much! Very clear! This technique is the same technique that disproves Darwinism. May I ask how to separate the sense strand from the antisense strand in the first step? what about covid-19? is it a mutagenesis? If we do this in pcr reaction chamber how can we seal up this plasmid into the circle one what about covid-19? sars cov2 gene sequence Love AK Lectures! Again getting me through my degree! :-) I don't understand how is the new DNA molecule circular again
Does the polymerase make it circular or you're adding a thermostable ligase to bound the resulting DNA molecule and make it circular? Informative ❤️ so useful! thank you very much the best videos ever honestly 10 minutes that summarizes a weeks worth of shitty lectures lol. Thanks bro! He is a great teacher. I would never be bumped to go to a class of him.